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李松洲 博士

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    聯絡人:唐維       資料來源:臨床醫學研究科       聯絡資訊:twei@vghks.gov.tw       

    基因體研究室研究員 
    李松洲 (Sung-Chou Li)

    學歷:

    1. 博士 陽明大學/中研院國際研究生學程 生物資訊學程 (2006/09~2011/03)
    2. 碩士 陽明大學 醫學生物技術所 (2003/09~2005/08)
    3. 學士 台灣大學 植物學系 (2020/09~2003/06)

    學術經歷:

    1. 高雄榮民總醫院 教學研究部研究員 (2023/03~)
    2. 高雄長庚紀念醫院 醫研部研究員 (2021/08~2023/03)
    3. 高雄長庚紀念醫院 醫研部副研究員 (2018/08~2021/07)
    4. 高雄長庚紀念醫院 醫研部助理研究員 (2014/08~2018/07)
    5. 高雄長庚紀念醫院 醫研部博士後研究員 (2013/04~2014/07)
    6. 中央研究院 基因體中心博士後研究員 (2011/04~2013/03)

    Art editor Img

    川崎症

    I am interested in vascular inflammation-related diseases which are characterized with leukocyte infiltration through the vein endothelial layer. To have a good experimental tool, since 2017 we have spent much time in developing the in vitro leukocyte-transendothelial migration (LTEM) assay to evaluate the severity of leukocyte infiltration. In 2018, by analyzing multiomics data (gene expression and DNA methylation) of Kawasaki disease (KD) patients, we found that S100A gene family were hypomethylated in DNA, enhancing the activities of S100A8/A9/A12 in KD. Using LTEM assay, we confirmed that the proteins of S100A8/A9/A12 promoted in vitro neutrophil infiltration through coronary artery endothelial cells, which was consistent with the mechanism of KD complication, coronary artery aneurysm (Clin Epigenetics. 2018 Nov 1;10(1):135). Using this LTEM assay, we also identified that miR-182-5p was a biomarker of KD complication and enhanced in vitro neutrophil infiltration in KD (Mol Genet Genomic Med. 2019 Dec;7(12):e990). In addition, by using iTRAQ gel-free proteomics, we analyzed serum samples and developed a serum protein-based KD biomarker panel, facilitating the timely prediction of Kawasaki disease. We also found that S100A12 enhanced the intensities of cell adhesion molecules of neutrophils. As a result, S100A12 promoted neutrophil infiltration which could be attenuated with S100A12 antibody treatment (Sci Rep. 2020 Sep 24;10(1):15645).

     

    ADHD

    Another research interest is attention-deficit/hyperactivity disorder (ADHD), which is diagnosed based on clinical manifestation without reliable molecular biomarkers. In 2018, we developed a miRNA biomarker panel to facilitate the prediction of ADHD (Front Psychiatry. 2018 May 29;9:227). We got the patent based on this technology in many countries. And this patent has been transferred to 賽亞基因 to develop commercial diagnosis kit. In 2019, we found that several of these miRNA biomarkers were associated with the gray matter volume (Eur Arch Psychiatry Clin Neurosci. 2020 Dec;270(8):1037-1045), implying their roles in neuron development. In 2022, by increasing the sample size, we confirmed our ADHD miRNA biomarker panel and enhanced the overall performance (Transl Psychiatry. 2022 Feb 19;12(1):67). In addition, with in vitro cell model, we concluded that miR-140-3p and miR-126-5p promoted the differentiation of neuron cells by repressing apoptosis and/or necrosis pathway.

    1. 專利名稱: 用於檢測川崎病的方法及套組

    許可專利之國家或地區: 中華民國 (I580788)、香港(1225759 B) 、中國(CN 106047991B)、

    2. 專利名稱: 用於偵測注意力缺陷/過動症之方法

    許可專利之國家或地區: 中華民國 (I690595)、法國(EP3369829-2018-09-05)、德國(60 2018 004 061.7)、歐盟(3369829)、中國(CN 108531571B)、

    3. 專利名稱: 蛋白質生物標記用以診斷川崎症的用途

    許可專利之國家或地區: 中華民國 (I704349)

    4. 2016年 第13屆 國家新創獎 運用核醣核酸開發川崎症分子診斷技術

    5. 2021年 第18屆 國家新創獎 全球第一套注意力缺失/過動症的分子診斷技術

    6. 2021創新技術博覽會發明競賽 用於偵測注意力缺陷/過動症之方法

    7. 技術移轉名稱: 用於檢測川崎病的方法及套組

    技術移轉廠商: 慧智基因股份有限公司

    8. 技術移轉名稱: 用於偵測注意力缺陷/過動症之方法

    技術移轉廠商: 賽亞基因科技股份有限公司

    2023: Journal of Physiology and Pharmacology, Journal of Obstetrics and Gynaecology Research, Microvascular Research, Tissue and Cell

    2022: Translational Psychiatry, BMC Genomics, Combinatorial Chemistry & High Throughput Screening, Epigenomics, BMC Pediatrics, Scientific Reports, Nutrients, Immunological Investigations,

    2021: Frontiers in Pediatrics, Medical Science Monitor, Combinatorial Chemistry & High Throughput Screening,

    2020: Biomarkers, Cardiovascular Therapeutics, Medical Science Monitor, Oxidative Medicine and Cellular Longevity

    2019: Epigenomics, International Journal of Molecular medicine, Frontiers in Oncology, European Journal of Pharmacology, Molecular Genetics and Genomic Medicine, Current Medicinal Chemistry, Archives of Medical Research, Molecular Medicine Reports, Immunological Investigation

    2018: Gene, Journal of Biomedical Science, Journal of Applied Biomedicine, Virus Research, BioMed Research International, Pathology-Research and Practice

    2017: Journal of Drug Targeting, Pathology-Research and Practice, Gene, eBioscience, Plos One, Virus Research

    2016: British Biotechnology Journal

    2015: Annual Research and Review in Biology

    2014: BMC Genomics, Computational Biology and Chemistry

    2013: Journal of Biomedical Science

    2012: Virology Journal

    2011: Marine Genomics

    2010: Bioinformatics, BMC Bioinformatics

    2009: FEBS

    2008: BMC Genomics

    (Selected publications from a total of 89)

    Complete list of publications: https://goo.gl/mKGOYh

     

    No.

    Article

    35

    Li SC, Lan KC, Hung HN, Huang WT, Lai YJ, Cheng HH, Tsai CC, Huang KL, You HL, Hsu TY: HSPA4 Is a Biomarker of Placenta Accreta and Enhances the Angiogenesis Ability of Vessel Endothelial Cells. Int J Mol Sci 2022, 23(10).

    34

    Wang LJ, Kuo HC, Lee SY, Huang LH, Lin Y, Lin PH, Li SC: MicroRNAs serve as prediction and treatment-response biomarkers of attention-deficit/hyperactivity disorder and promote the differentiation of neuronal cells by repressing the apoptosis pathway. Transl Psychiatry 2022, 12(1):67.

    33

    Wang LJ, Li SC, Li SW, Kuo HC, Lee SY, Huang LH, Chin CY, Yang CY: Gut microbiota and plasma cytokine levels in patients with attention-deficit/hyperactivity disorder. Transl Psychiatry 2022, 12(1):76.

    32

    Wang LJ, Huang YC, Lin PY, Lee Y, Hung CF, Hsu ST, Huang LH, Li SC: BST-1 as a serum protein biomarker involved in neutrophil infiltration in schizophrenia. World J Biol Psychiatry 2021:1-11.

    31

    Yu HR, Hsu TY, Tsai CC, Huang HC, Cheng HH, Lai YJ, Lin YJ, Chen CC, Li SC, Yang K: The Functional DNA Methylation Signatures Relevant to Altered Immune Response of Neonatal T Cells with l-Arginine Supplementation. Nutrients 2021, 13(8).

    30

    Li SC, Kuo HC, Huang LH, Chou WJ, Lee SY, Chan WC, Wang LJ: DNA Methylation in LIME1 and SPTBN2 Genes Is Associated with Attention Deficit in Children. Children (Basel) 2021 Jan 29;8(2):92.

    29

    Li SC, Tsai KW, Huang LH, Weng KP, Chien KJ, Lin Y, Tu CY, Lin PH: Serum proteins may facilitate the identification of Kawasaki disease and promote in vitro neutrophil infiltration. Sci Rep 2020, 10(1):15645.

    28

    Hsu TY, Tsai KW, Lan KC, Hung HN, Lai YJ, Cheng HH, Tsai CC, Li SC: Identifying the potential protein biomarkers of preterm birth in amniotic fluid. Taiwan J Obstet Gynecol 2020, 59(3):366-371.

    27

    Tsai MH, Lin CH, Tsai KW, Lin MH, Ho CJ, Lu YT, Weng KP, Lin Y, Lin PH, Li SC: S100A6 Promotes B Lymphocyte Penetration Through the Blood–Brain Barrier in Autoimmune Encephalitis. Front Genet. 2019 Nov 22;10:1188. doi: 10.3389/fgene.2019.01188. eCollection 2019.

    26

    Kuo HC, Pan CT, Huang YH, Huang FC, Lin YS, Li SC, Huang LH: Global Investigation of Immune Repertoire Suggests Kawasaki Disease Has Infectious Cause. Circ J. 2019 Sep 25;83(10):2070-2078. doi: 10.1253/circj.CJ-19-0206

    25

    Li SC, Huang LH, Chien KJ, Pan CY, Lin PH, Lin Y, Weng KP, Tsai KW: MiR-182-5p enhances in vitro neutrophil infiltration in Kawasaki disease. Mol Genet Genomic Med 2019:e990.

    24

    Wang LJ, Yang CY, Chou WJ, Lee MJ, Chou MC, Kuo HC, Yeh YM, Lee SY, Huang LH, Li SC: Gut microbiota and dietary patterns in children with attention-deficit/hyperactivity disorder. Eur Child Adolesc Psychiatry 2019 May 22. doi: 10.1007/s00787-019-01352-2

    23

    Yang CM, Chang HS, Chen HC, You JJ, Liou HH, Ger LP, Li SC, Tsai KW: Low C6orf141 Expression Is Significantly Associated With a Poor Prognosis in Patients With Oral Cancer. Sci Rep 2019, 9(1):4520.

    22

    Huang LH, Kuo HC, Pan CT, Lin YS, Huang YH, Li SC: Multiomics analyses identified epigenetic modulation of the S100A gene family in Kawasaki disease and their significant involvement in neutrophil transendothelial migration. Clin Epigenetics 2018, 10(1):135.

    21

    Yu HR, Huang LH, Li SC. Roles of microRNA in the immature immune system of neonates. Cancer Lett. 2018 Oct 1;433:99-106

    20

    Wang LJ, Li SC, Lee MJ, Chou MC, Chou WJ, Lee SY, Hsu CW, Huang LH, Kuo HC.: Blood-Bourne MicroRNA Biomarker Evaluation in Attention-Deficit/Hyperactivity Disorder of Han Chinese Individuals: An Exploratory Study. Front Psychiatry. 2018 May 29;9:227.

    19

    Huang LH, Lin PH, Tsai KW, Wang LJ, Huang YH, Kuo HC, Li SC: The effects of storage temperature and duration of blood samples on DNA and RNA qualities. PLoS One 2017, 12(9):e0184692.

    18

    Li SC, Wang FS, Yang YL, Tiao MM, Chuang JH, Huang YH:

    Microarray Study of Pathway Analysis Expression Profile Associated with MicroRNA-29a with Regard to Murine Cholestatic Liver Injuries. Int J Mol Sci 2016, 17(3).

    17

    Li SC, Chan WC, Huang YH, Guo MM, Yu HR, Huang FC, Kuo HC: Major methylation alterations on the CpG markers of inflammatory immune associated genes after IVIG treatment in Kawasaki disease. BMC Med Genomics 2016, 9 Suppl 1:37.

    16

    Kuo HC, Li SC, Guo MM, Huang YH, Yu HR, Huang FC, Jiao F, Andrade J, Chan WC: Genome-Wide Association Study Identifies Novel Susceptibility Genes Associated with Coronary Artery Aneurysm Formation in Kawasaki Disease.

    PLoS One 2016, 11(5):e0154943

    15

    Kuo HC, Hsieh KS, Ming-Huey Guo M, Weng KP, Ger LP, Chan WC, Li SC: Next-generation sequencing identifies micro-RNA-based biomarker panel for Kawasaki disease. J Allergy Clin Immunol 2016, 138(4):1227-1230.

    14

    Tsai KW, Chang B, Pan CT, Lin WC, Chen TW, Li SC: Evaluation and application of the strand-specific protocol for next-generation sequencing. Biomed Res Int 2015, 2015:182389.

    13

    Pan CT, Tsai KW, Hung TM, Lin WC, Pan CY, Yu HR, Li SC: miRSeq: A User-Friendly Standalone Toolkit for Sequencing Quality Evaluation and miRNA Profiling. Biomed Res Int 2014, 2014:462135.

    12

    Li SC, Tsai KW, Pan HW, Jeng YM, Ho MR, Li WH: MicroRNA 3' end nucleotide modification patterns and arm selection preference in liver tissues. BMC Syst Biol 2012, 6 Suppl 2:S14.

    11

    Li SC, Liao YL, Ho MR, Tsai KW, Lai CH, Lin WC: miRNA arm selection and isomiR distribution in gastric cancer. BMC Genomics 2012, 13 Suppl 1:S13.

    10

    Chang HT, Li SC, Ho MR, Pan HW, Ger LP, Hu LY, Yu SY, Li WH, Tsai KW: Comprehensive analysis of microRNAs in breast cancer. BMC Genomics 2012, 13 Suppl 7:S18.

    9

    Li SC, Liao YL, Chan WC, Ho MR, Tsai KW, Hu LY, Lai CH, Hsu CN, Lin WC: Interrogation of rabbit miRNAs and their isomiRs. Genomics 2011, 98(6):453-459.

    8

    Li SC, Chan WC, Lai CH, Tsai KW, Hsu CN, Jou YS, Chen HC, Chen CH, Lin WC: UMARS: Un-MAppable Reads Solution. BMC Bioinformatics 2011, 12 Suppl 1:S9.

    7

    Li SC, Chan WC, Hu LY, Lai CH, Hsu CN, Lin WC: Identification of homologous microRNAs in 56 animal genomes. Genomics 2010, 96(1):1-9.

    6

    Li SC, Chan WC, Ho MR, Tsai KW, Hu LY, Lai CH, Hsu CN, Hwang PP, Lin WC: Discovery and characterization of medaka miRNA genes by next generation sequencing platform. BMC Genomics 2010, 11 Suppl 4:S8.

    5

    Lin WC, Li SC, Shin JW, Hu SN, Yu XM, Huang TY, Chen SC, Chen HC, Chen SJ, Huang PJ et al: Identification of microRNA in the protist Trichomonas vaginalis. Genomics 2009, 93(5):487-493.

    4

    Yu X, Zhou Q, Li SC, Luo Q, Cai Y, Lin WC, Chen H, Yang Y, Hu S, Yu J: The silkworm (Bombyx mori) microRNAs and their expressions in multiple developmental stages. PLoS ONE 2008, 3(8):e2997.

    3

    Li SC, Shiau CK, Lin WC: Vir-Mir db: prediction of viral microRNA candidate hairpins. Nucleic Acids Res 2008, 36(Database issue):D184-189.

    2

    Li SC, Tang P, Lin WC: Intronic MicroRNA: Discovery and Biological Implications. DNA Cell Biol 2007, 26(4):195-207.

    1

    Li SC, Pan CY, Lin WC: Bioinformatic discovery of microRNA precursors from human ESTs and introns. BMC Genomics 2006, 7:164.

     
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